Imaging Cytometry Unit

Microscopy is extensively used in almost every field of contemporary biology.
Researchers at the Faculty currently share one standard confocal microscope with limited capabilities. Current investment will enable them to use new apparatus in researches focusing on biochemistry, biophysics and molecular biology e.g studies on DNA damage and repair, DNA-drug interaction, oxidative stress, hypoxia, protein subcellular localization, protein – ligand interaction), cell migration and many others.		    

Modernized laboratory will enable research on:

  • mobility, dynamic exchange and diffusion of fluorescently labeled proteins with FRAP techniques (fast changes of inhaled light intensity, possibility of causing photofading in any area of any shape);
  • fast detection of fluorescence (in split seconds after fading termination), that gives the possibility of registering shape of fluorescence recovery curve in the initial phase, which is significant for marking out FRAP parameters;
  • labeled proteins diffusion with FCS method and interactions between proteins in situ with FRAP method;
  • interactions between cell and matrix in tissues and tissue-modelling systems, for example in multicellular spheroids (multiphoton excitation);
  • photoactivation of fluorescent proteins during chromatin reorganization.

Purchased equipment

Team leaded by prof. Dobrucki together with team of prof Zbigniew Dażynkiewicz (New York Medical College) has developed a new fluorescent marker that allows direct staining of collagens. This will allow observation of collagen distribution / localisation in tissues and will facilitate reaserch on multiple biological processess (e.g. aging) and development of new medicinal products

"Thanks to that that invention, we will be able to study living, isolated but not fixated and not damaged tissues" - said prof. Dobrucki (PAP)

More information

 

Person in charge:

Jerzy Dobrucki, PhD, DSc


Head of Cell Biophysics Laboratory FBBB
Deputy dean for International Cooperation and Science, FBBB UJ More information: Cell biophysics department, JU